Project-JRF Recruitment – Structural & Functional Analysis of FlhF

Project-JRF Recruitment – Structural & Functional Analysis of FlhF

“Structural and Functional Analysis of FlhF: A Signal Recognition Particle (SRP) Type GTPase Involved in Flagellar Localization in Pseudomonas aeruginosa”

This project aims to investigate FlhF, a highly conserved SRP-type GTPase that plays a crucial role in determining the correct number and spatial placement of flagella in Pseudomonas aeruginosa. FlhF is known to regulate flagellar biogenesis, ensuring that the flagellum is positioned at the appropriate cellular pole, which is essential for effective motility, host colonization, and pathogenicity.

The research involves understanding both the structural features and biochemical functions of FlhF. By examining its GTP-binding properties, interaction partners, and three-dimensional structure, the project aims to uncover how FlhF coordinates flagellar assembly at the molecular level.

This includes:

• Determining high-resolution structural details using methods such as X-ray crystallography or biophysical techniques.
• Performing protein purification, biochemical assays, and crystallization trials to characterize FlhF’s activity.
• Exploring how structural variations influence its GTPase function and role in flagellar localization.

A deeper understanding of FlhF will provide insights into the mechanisms of bacterial motility, potentially revealing new antimicrobial targets or strategies to interfere with the motility-dependent virulence of Pseudomonas aeruginosa.

JRF Recruitment :

Project Junior Research Fellow (Project-JRF)
Location: Regional Centre for Biotechnology (RCB), Faridabad
Principal Investigator: Prof. Deepti Jain
Number of Positions: 01 Time Duration : 01
Age Limit : Maximum 30 years (as on 30 November 2025)
Fellowship / Salary : ₹37,000 + 16% HRA per month

Eligibility Criteria :

Essential Qualification

• M.Sc. in Life Sciences with NET / DBT-JRF / GATE / ICMR qualification.
• Minimum 60% marks in Master’s degree or equivalent.

Essential Experience

• Hands-on experience in:
  • Protein purification
  • Biochemical assays
  • Protein crystallization

Desirable Expertise

• Experience in:
  • X-ray crystallography
  • Structural biology
  • Biophysics laboratory techniques
• Good oral and written communication skills

How to Apply

• Interested candidates must apply online by 10 December 2025 (5:00 PM).
• Applicants must upload all relevant certificates, including proof of experience in the desired research areas.

Selection Process

• Shortlisted candidates will be notified by email.
• Interview Date: 15 December 2025
• Reporting Time: 10:00 AM onwards

Contact Us :

For queries, email the Principal Investigator:
📧 deepti@rcb.res.in

Documents to Upload

1. Filled application form
2. Updated and detailed CV
3. M.Sc. Marksheet (Life Sciences/Biotechnology)
4. Certificate of qualifying NET/DBT-JRF/GATE/ICMR